Identification of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma.

Identification of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma.

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Recently, there has been renewed interest in the role of tumor stem cells (TSCs) in tumorigenesis, chemoresistance, and relapse of malignant tumors including osteosarcoma.The potential exists to improve osteosarcoma treatment through characterization of TSCs and identification of therapeutic targets.Using transcriptome, proteome, immunophenotyping for cell-surface markers, and bioinformatic analyses, heterogeneous expression of previously reported TSC Reducing Cognitive Load and Improving Warfighter Problem Solving With Intelligent Virtual Assistants or osteosarcoma markers, such as CD133, nestin, POU5F1 (OCT3/4), NANOG, SOX2, and aldehyde dehydrogenase, among others, was observed in vitro.

However, consistently significantly lower CD326, CD24, CD44, and higher ABCG2 expression in TSC-enriched as compared with un-enriched osteosarcoma cultures was observed.In addition, consistently higher CBX3 expression in TSC-enriched osteosarcoma cultures was identified.ABCA5 was identified as a putative biomarker of TSCs and/or osteosarcoma.

Lastly, in a high-throughput screen we identified epigenetic (5-azacytidine), anti-microtubule (vincristine), and Atlantic Niño induced sea surface salinity variability as observed from the satellite anti-telomerase (3,11-difluoro-6,8,13-trimethyl- 8H-quino [4,3,2-kl] acridinium methosulfate; RHPS4)-targeted therapeutic agents as candidates for TSC ablation in osteosarcoma.